![]() There are several reasons why you might want to make a spore print. Usually white paper is used for spore prints, but white spore prints may be difficult to see and some people have chosen to make spore prints of a mushroom on both black and white paper. It is a photograph-like impression of the pileus and lamellae. The spores produced on the lamellae had been discharged during the night and had formed a thick deposit on the paper, a result called a spore print. The same pileus that had been removed from its stipe was place lamella-side down on a sheet of white paper, covered with a bowl and left overnight. At far left is a scan of the same pileus inverted to show the lamellae. The leftmost mushroom in the picture had its stipe removed and placed beside the pileus. These were "photographed" on a flatbed scanner not long after they were collected. This method is however a good solid foundation that will undoubtedly yield a great success rate and excellent results.The picture above is of four fruiting bodies of Cortinarius armillatus, a mushroom commonly collected under birch. There are several variations of this method, some of which use more advanced equipment and containers to increase the chances of your mixture staying sterile. This is one method that can be used to make a mushroom spore syringe. Once properly stored, your spore syringes will remain viable for 2-6 months. #SPORE PRINT MUSHROOM ZIP#After 2-3 days, store your syringe in an airtight zip lock bag or in a fridge for future use.Once you’ve filled your syringe, leave it at room temperature for 2-3 days in order for the spores to fully hydrate.Do this every time you add more mushroom spores to the water. This will ensure that there is a good spread of spores throughout the water. It is a good idea to empty the syringe back into the beaker, and to repeat this process a few times. Fill your syringe instantly by placing the tip of your first syringe in the water and filling it up as soon as you’ve scraped the spores into the water.Use your sterilized scalpel to carefully scrape some of the spores into the water.Using your sterilized tweezers, remove the spore print from its storage and hold it over the opening of the beaker.Remove the tin foil you placed over your beaker.Start by sterilizing your tweezers and scalpel in a flame. Once the cooling process is completed, you are ready to make your spore syringe.A good idea is to let the water sit overnight. It will take a few hours for the water to cool down to room temperature. If you proceed while the water is still hot, you run a big risk of killing the mushroom spores. This is a vital step in the process of making your spore syringe. After 30 minutes, turn off the heat completely and allow the water to cool to room temperature.Once 15psi is reached, lower the temperature of your pressure cooker so that it is maintained for approximately 30 minutes. Next, place your beaker/bottle in the pressure cooker and heat it until it reaches 15psi.Once sterilized, fill your beaker halfway with water and cover it with tin foil to prevent any air from entering the beaker. The first step in making your spore syringe is to sterilize your water and glass beaker.A beaker or a narrow-necked glass flask.You will need the following to make a spore syringe: As the spores in spore syringes are naturally hydrated and ready to go, spore syringes are very convenient to use. This makes it a vital process before mushroom breeding can begin. The hydration of your spores is incredibly important as dry spores are highly unlikely to germinate. A spore syringe contains sterile water containing hydrated spores, which is referred to as spore solution. The process of making a spore syringe is relatively simple. It is essential that you minimize contamination from other fungi spores or bacteria. Before you start making your spore syringe, it is important to ensure that the space you are making it in is clean and sterile. ![]()
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